The Effect of Antibody Concentration on Pilator Sensitivity (Rapid Salmonella Detector) as a Colourimetric-Based Salmonella Detection Biosensor
DOI:
https://doi.org/10.47494/mesb.2022.31.1763Keywords:
Biosensor, Immunosensor, PILATOR, Salmonella, AntibodyAbstract
Food safety is one of the essential basics of human rights. Food should be the source of nourishment for human beings and not an open door for potentially pathogenic bacteria to avoid foodborne disease. One of the most pathogenic bacteria is Salmonella. This study aims to know the effect of antibody concentration on PILATOR (Rapid Salmonella Detector) uses the descriptive method. The results can detect Salmonella in 10 minutes, with the optimum concentration of primary and secondary antibodies being 10 μg ml-1 and 0.2 μg ml-1. Based on time detection (10 minutes) and 102 CFU/ml limit detection. PILATOR is categorized as a fast and accurate Salmonella detector.
Downloads
References
J. Sutejo, “Pengaruh Pendidikan Kesehatan Tentang Penyakit Bawaan Makanan Terhadap Pengetahuan Siswa/I Kelas IV, V Dan VI Mengenai Penyakit Bawaan Makanan Disekolah Dasar 060929 Medan Johor.,” Universitas Sumatera Utara, 2016.
A. Nugraha, “Deteksi Bakteri Salmonella spp. dan Pengujian Kualitas Telur Ayam Buras,” J. Indones. Med. Veterinus, vol. 1, no. 3, pp. 320–329, 2012.
J. Odomeru and Verlade, Salmonella Detection Methods for Food and Food Ingredients. Ontario, Canada: University of Guelph, 2012.
J. Bueno, “Bisensors in Antimicrobial Drug Discovery: Since Biology Until Screening Platform,” Microb. Biochem. Technol., pp. 1–10, 2014.
A. Koyun, A. Esma, and Yeliz., “Biosensor and Their Principle, A Roadmap of Biomedical Engineers and Milistone,” Turkey Tech Ollesgun, 2012.
N. Lipman, L. Jackson, L. Trudel, and Weis-Garcia, “Monoclonal Versus Polyclonal Antibodies: Distinguishing Characteristics, Applications, and Information Resources,” ILAR J. Vol., vol. 46, no. 3, 2005.
Thermo Scientific, ELISA Technical Guide and Protocols. Rockford: Thermo Scientific, 2010.
F. Salam and I. E. Tothill, “Detection of Salmonella typhimurium using an electrochemical immunosensor,” Biosens. Bioelectron., vol. 24, no. 8, pp. 2630–2636, Apr. 2009, doi: 10.1016/j.bios.2009.01.025.
R. Cao, L. Guan, M. Li, J. Tian, and W. Shen, “A zero-step functionalization on paper-based biosensing platform for covalent biomolecule immobilization,” Sens. Bio-Sensing Res., vol. 6, pp. 13–18, Dec. 2015, doi: 10.1016/j.sbsr.2015.09.002.
M. Zourob, Principles of Bacterial Detection: Biosensors, Recognition Receptors and Microsystem. New York: Springer Science & Business Media, 2008.
RnD System, ELISA Development Guide. Minneapolis: R&D Systems Inc, 2002.
Published
How to Cite
Issue
Section
Copyright (c) 2022 Yunita Khilyatun Nisak
This work is licensed under a Creative Commons Attribution 4.0 International License.
The work simultaneously licensed under a Creative Commons Attribution 4.0 International License
You are free to:
- Share — copy and redistribute the material in any medium or format
- Adapt — remix, transform, and build upon the material for any purpose, even commercially.
The licensor cannot revoke these freedoms as long as you follow the license terms.
Under the following terms:
-
Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
- No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.
