Application of Chromatography in Medicine
Keywords:chromatographic separation technique, thin layer chromatography, High-performance liquid chromatography, elution time, applications
Chromatography is a separation process used to separate components in a mixture. The components of the mixture are dispersed in a liquid solution known as the mobile phase., which holds it through a structure containing another substance known as the stationary phase. Component separation requires differential partitioning between the mobile and stationary phases. The analytical goal of chromatography is to determine the qualitative and quantitative chemical makeup of a sample, and its primary purpose is to purify and extract one or more components of a sample. This paper will discuss the history and basics of what chromatography is meant and the main principles of how we can run it. besides, we will mention and focus on an application for each chromatographic type such as planar, TLC, gas, liquid separation techniques.
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